The flow cytometrc DNA analysis and the immunohistochemical staining of proli-ferating cell nuclear antigen (PCNA) & c-H-ras oncogene have been reported t reflect the cellular activities and the proliferative rates of the progression of
neoplasms.
The DNA contents and cell cycles of 56 cases of colon adenocarcinoma resected in Korea University Hospital were measured by flow cytometry in order to evaluate the relationship between the expression of c-H-ras oncogene & PCNA.
@ES The results were summarized as follws;
@EN 1. There were no significant differences between the ploidy pattern and histolo-gic type (X2=2.89, 2df, p=0.235), between the ploidy pattern and modified Dukes classification (X2=6.74, 3df, p=0.08).
2. There were significant differences between the diploid and aneuplid in Go+G1 phase (p<0.01), S phase (p<0.01) and proliferating index(PI)(p<0.05).
3. G2+M/G0+G1 was significantly higher in C1 than B1, G2 and C2(p<0.05), G1+G1 and S phase was also significantly higher in poorly than well differntiated type (p<0.05).
4. 4. The strong positive correlation between the staining index of c-H-ras oncoge-ne and PI was noted in the aneuploidy pattern of colon adenocarcinoma (r=0.66, p<0.05).
5. There was positive correlation between the staining index of PCNA and the st-aining index of c-H-ras oncogene(r=0.40, p<0.01).
6. We could not observe the increase of S phase & PI which reflect the prolifer-ative activity in spite of increasing in staining index of PCNA and c-H-ras oncogene. (S=11.07-2.65 SPI+4.51 CSI, Rsquare=0.06, P of PSI=0.39, P of CSI=0.09.
PI=12.92+1.14
PSI+4.26 CSI, R square=0.06, P of CSI=0.76, P of CSI=0.24).
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